Determining the oligomeric state of mitochondrial carrier proteins by blue native gel electrophoresis
نویسندگان
چکیده
منابع مشابه
Oligomeric state of membrane transport proteins analyzed with blue native electrophoresis and analytical ultracentrifugation.
Blue native electrophoresis is used widely for the analysis of non-dissociated protein complexes with respect to composition, oligomeric state and molecular mass. However, the effects of detergent or dye binding on the mass and stability of the integral membrane proteins have not been studied. By comparison with analytical ultracentrifugation, we have evaluated whether the oligomeric state of m...
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The two-dimensional blue native/sodium dodecyl sulfate polyacrylamide gel electrophoresis (2D BN/SDS-PAGE) is a method of choice for the investigation of protein complexes. This highly resolvent separation method is unique in that it facilitates the identification of many protein complexes simultaneously. Because of its simplicity and suitability, the 2D BN/SDS-PAGE can be now applied to a wide...
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Blue-native (BN) PAGE is a powerful tool for isolation and separation of intact membrane protein complexes. In the first dimension, monomeric and multimeric protein complexes are separated in their native state. A subsequent SDS PAGE step is used to separate the complex subunits. We applied this method to analyze the formation and stability of yeast mitochondrial complexes and supercomplexes us...
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Oxidized nicotinamide adenine dinucleotide (NAD(+)) kinase (NADK, E.C. 2.7.1.23) plays an instrumental role in cellular metabolism. Here we report on a blue native polyacrylamide gel electrophoretic technique that allows the facile detection of this enzyme. The product, oxidized nicotinamide adenine dinucleotide phosphate (NADP(+)), formed following the reaction of NADK with NAD(+) and adenosin...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Bioenergetics
سال: 2010
ISSN: 0005-2728
DOI: 10.1016/j.bbabio.2010.04.146